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primer express 3.0 software  (Thermo Fisher)


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    Thermo Fisher primer express 3.0 software
    Primer Express 3.0 Software, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primer+express+3%2E0+software/primer+express+software/pm40490239-92-8-12
    Average 90 stars, based on 1 article reviews
    primer express 3.0 software - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Quantitative RT-PCR:

    Article Title: Injury-induced KIF4A neural expression and its role in Schwann cell proliferation suggest a dual function for this kinesin in neural regeneration
    Article Snippet: The Rattus norvegicus Kif4a (NM_001400930.1) and Odc (NM_012615.3) reference sequences were applied to design specific primer pairs (Primer Express 3.0 Software, Applied Biosystems): TCC GGG AGG ATC CTA AGG AA (Kif4a fwd) and GTC CGA GGC AAC TAG CAC AGT (Kif4a rev), TTG ACC AGA TGA ACG GAG TG (Krox20 fwd) and CAG AGA TGG GAG CGA AGC TA (Krox20 rev), GTT CTC GCA GAC CAC CAT CT (Oct6 fwd) and GTC TCC TCC AGC CAC TTG TT (Oct6 rev), GGT TCC AGA GGC CAA ACA TC (Odc fwd) and GTT GCC ACA TTG ACC GTG AC (Odc rev), GAA CGG GAA GCT CAC TGG C (Gapdh fwd) and GCA TGT CAG ATC CAC AAC G (Gapdh rev).

    Article Title: Biosynthesis of Phenolic Compounds of Medicago truncatula After Inoculation with Selected PGPR Strains
    Article Snippet: PCR primers for both semi-quantitative and quantitative methods were designed using Primer Express 3.0 software (Applied Biosystems, Carlsbad, CA, USA)— .

    Article Title: Structural and functional plasticity of metal-responsive transcription factor 1 (MTF-1) within Lophotrochozoa.
    Article Snippet: Control of metal ion metabolism in animals is crucial for their survival.. In this context, the regulation of metallothioneins (MTs), key proteins in metal metabolism and detoxification, by the metal regulatory transcription factor MTF-1 is well established in vertebrates and Drosophila melanogaster.. However, the metal-dependent regulation of MT genes in most other invertebrates remains poorly understood, with MTF-1 so far identified in only a few invertebrate species.

    Article Title: Stress-induced hyperglycemia and expression of glucose cell transport genes in skeletal muscle of critically ill patients: a cross-sectional study
    Article Snippet: Specific primers for IRS1, IRS2, SLC2A1 , and SLC2A4 were designed using published human sequences and Primer Express 3.0 software (Thermo Fisher Scientific). contains the sequence information for all primers.

    Article Title: Transcriptional responses of Metarhizium pingshaense blastospores after UV-B irradiation
    Article Snippet: The primers were designed using Primer Express 3.0 software (Thermo Fisher Scientific, Wilmington, DE, USA) and the transcripts obtained 1 https://www.ncbi.nlm.nih.gov/sra/?term=prjna1127190 Frontiers in Microbiology 04 frontiersin.org from the transcriptome itself (Supplementary Table 1).

    Article Title: Transcriptional responses of Metarhizium pingshaense blastospores after UV-B irradiation
    Article Snippet: The primers were designed using Primer Express 3.0 software (Thermo Fisher Scientific, Wilmington, DE, USA) and the transcripts obtained from the transcriptome itself ( ).

    Article Title: Molecular Biodiversity in Fusarium subglutinans and F. temperatum : A Valuable Tool to Distinguish the Two Sister Species and Determine the Beauvericin Chemotype
    Article Snippet: Species-specific primers were designed using Primer Express 3.0 software (Applied Biosystem, Forster City, CA, USA).

    Article Title: Multi-omics analysis reveals the positive impact of differential chloroplast activity during in vitro regeneration of barley.
    Article Snippet: 1 Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India 2 Institute of Molecular Plant Biology, ETH Zurich, Zurich, Switzerland 3 Department of Chemistry, Biochemistry and Physics, University of Quebec Trois Rivieres, Trois Rivieres, QC, Canada 4 Present address: University Institute of Biotechnology, Chandigarh University, Punjab 140413, India Abstract Existence of potent in vitro regeneration system is a prerequisite for efficient genetic transformation and functional genomics of crop plants.. In this study, two contrasting cultivars differencing in their in vitro regeneration efficiency were identified.. Tissue culture friendly cultivar Golden Promise (GP) and tissue culture resistant DWRB91(D91) were selected as contrasting cultivars to investigate the molecular basis of regeneration efficiency through multiomics analysis.

    Software:

    Article Title: Injury-induced KIF4A neural expression and its role in Schwann cell proliferation suggest a dual function for this kinesin in neural regeneration
    Article Snippet: The Rattus norvegicus Kif4a (NM_001400930.1) and Odc (NM_012615.3) reference sequences were applied to design specific primer pairs (Primer Express 3.0 Software, Applied Biosystems): TCC GGG AGG ATC CTA AGG AA (Kif4a fwd) and GTC CGA GGC AAC TAG CAC AGT (Kif4a rev), TTG ACC AGA TGA ACG GAG TG (Krox20 fwd) and CAG AGA TGG GAG CGA AGC TA (Krox20 rev), GTT CTC GCA GAC CAC CAT CT (Oct6 fwd) and GTC TCC TCC AGC CAC TTG TT (Oct6 rev), GGT TCC AGA GGC CAA ACA TC (Odc fwd) and GTT GCC ACA TTG ACC GTG AC (Odc rev), GAA CGG GAA GCT CAC TGG C (Gapdh fwd) and GCA TGT CAG ATC CAC AAC G (Gapdh rev).

    Article Title: Biosynthesis of Phenolic Compounds of Medicago truncatula After Inoculation with Selected PGPR Strains
    Article Snippet: PCR primers for both semi-quantitative and quantitative methods were designed using Primer Express 3.0 software (Applied Biosystems, Carlsbad, CA, USA)— .

    Article Title: Structural and functional plasticity of metal-responsive transcription factor 1 (MTF-1) within Lophotrochozoa.
    Article Snippet: Control of metal ion metabolism in animals is crucial for their survival.. In this context, the regulation of metallothioneins (MTs), key proteins in metal metabolism and detoxification, by the metal regulatory transcription factor MTF-1 is well established in vertebrates and Drosophila melanogaster.. However, the metal-dependent regulation of MT genes in most other invertebrates remains poorly understood, with MTF-1 so far identified in only a few invertebrate species.

    Article Title: Stress-induced hyperglycemia and expression of glucose cell transport genes in skeletal muscle of critically ill patients: a cross-sectional study
    Article Snippet: Specific primers for IRS1, IRS2, SLC2A1 , and SLC2A4 were designed using published human sequences and Primer Express 3.0 software (Thermo Fisher Scientific). contains the sequence information for all primers.

    Article Title: Transcriptional responses of Metarhizium pingshaense blastospores after UV-B irradiation
    Article Snippet: The primers were designed using Primer Express 3.0 software (Thermo Fisher Scientific, Wilmington, DE, USA) and the transcripts obtained 1 https://www.ncbi.nlm.nih.gov/sra/?term=prjna1127190 Frontiers in Microbiology 04 frontiersin.org from the transcriptome itself (Supplementary Table 1).

    Article Title: Transcriptional responses of Metarhizium pingshaense blastospores after UV-B irradiation
    Article Snippet: The primers were designed using Primer Express 3.0 software (Thermo Fisher Scientific, Wilmington, DE, USA) and the transcripts obtained from the transcriptome itself ( ).

    Article Title: Molecular Biodiversity in Fusarium subglutinans and F. temperatum : A Valuable Tool to Distinguish the Two Sister Species and Determine the Beauvericin Chemotype
    Article Snippet: Species-specific primers were designed using Primer Express 3.0 software (Applied Biosystem, Forster City, CA, USA).

    Article Title: Multi-omics analysis reveals the positive impact of differential chloroplast activity during in vitro regeneration of barley.
    Article Snippet: 1 Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India 2 Institute of Molecular Plant Biology, ETH Zurich, Zurich, Switzerland 3 Department of Chemistry, Biochemistry and Physics, University of Quebec Trois Rivieres, Trois Rivieres, QC, Canada 4 Present address: University Institute of Biotechnology, Chandigarh University, Punjab 140413, India Abstract Existence of potent in vitro regeneration system is a prerequisite for efficient genetic transformation and functional genomics of crop plants.. In this study, two contrasting cultivars differencing in their in vitro regeneration efficiency were identified.. Tissue culture friendly cultivar Golden Promise (GP) and tissue culture resistant DWRB91(D91) were selected as contrasting cultivars to investigate the molecular basis of regeneration efficiency through multiomics analysis.

    Sequencing:

    Article Title: Injury-induced KIF4A neural expression and its role in Schwann cell proliferation suggest a dual function for this kinesin in neural regeneration
    Article Snippet: The Rattus norvegicus Kif4a (NM_001400930.1) and Odc (NM_012615.3) reference sequences were applied to design specific primer pairs (Primer Express 3.0 Software, Applied Biosystems): TCC GGG AGG ATC CTA AGG AA (Kif4a fwd) and GTC CGA GGC AAC TAG CAC AGT (Kif4a rev), TTG ACC AGA TGA ACG GAG TG (Krox20 fwd) and CAG AGA TGG GAG CGA AGC TA (Krox20 rev), GTT CTC GCA GAC CAC CAT CT (Oct6 fwd) and GTC TCC TCC AGC CAC TTG TT (Oct6 rev), GGT TCC AGA GGC CAA ACA TC (Odc fwd) and GTT GCC ACA TTG ACC GTG AC (Odc rev), GAA CGG GAA GCT CAC TGG C (Gapdh fwd) and GCA TGT CAG ATC CAC AAC G (Gapdh rev).

    Article Title: Biosynthesis of Phenolic Compounds of Medicago truncatula After Inoculation with Selected PGPR Strains
    Article Snippet: PCR primers for both semi-quantitative and quantitative methods were designed using Primer Express 3.0 software (Applied Biosystems, Carlsbad, CA, USA)— .

    Article Title: Structural and functional plasticity of metal-responsive transcription factor 1 (MTF-1) within Lophotrochozoa.
    Article Snippet: Control of metal ion metabolism in animals is crucial for their survival.. In this context, the regulation of metallothioneins (MTs), key proteins in metal metabolism and detoxification, by the metal regulatory transcription factor MTF-1 is well established in vertebrates and Drosophila melanogaster.. However, the metal-dependent regulation of MT genes in most other invertebrates remains poorly understood, with MTF-1 so far identified in only a few invertebrate species.

    Article Title: Stress-induced hyperglycemia and expression of glucose cell transport genes in skeletal muscle of critically ill patients: a cross-sectional study
    Article Snippet: Specific primers for IRS1, IRS2, SLC2A1 , and SLC2A4 were designed using published human sequences and Primer Express 3.0 software (Thermo Fisher Scientific). contains the sequence information for all primers.

    Article Title: Transcriptional responses of Metarhizium pingshaense blastospores after UV-B irradiation
    Article Snippet: The primers were designed using Primer Express 3.0 software (Thermo Fisher Scientific, Wilmington, DE, USA) and the transcripts obtained 1 https://www.ncbi.nlm.nih.gov/sra/?term=prjna1127190 Frontiers in Microbiology 04 frontiersin.org from the transcriptome itself (Supplementary Table 1).

    Article Title: Transcriptional responses of Metarhizium pingshaense blastospores after UV-B irradiation
    Article Snippet: The primers were designed using Primer Express 3.0 software (Thermo Fisher Scientific, Wilmington, DE, USA) and the transcripts obtained from the transcriptome itself ( ).

    Article Title: Molecular Biodiversity in Fusarium subglutinans and F. temperatum : A Valuable Tool to Distinguish the Two Sister Species and Determine the Beauvericin Chemotype
    Article Snippet: Species-specific primers were designed using Primer Express 3.0 software (Applied Biosystem, Forster City, CA, USA).

    Article Title: Multi-omics analysis reveals the positive impact of differential chloroplast activity during in vitro regeneration of barley.
    Article Snippet: 1 Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India 2 Institute of Molecular Plant Biology, ETH Zurich, Zurich, Switzerland 3 Department of Chemistry, Biochemistry and Physics, University of Quebec Trois Rivieres, Trois Rivieres, QC, Canada 4 Present address: University Institute of Biotechnology, Chandigarh University, Punjab 140413, India Abstract Existence of potent in vitro regeneration system is a prerequisite for efficient genetic transformation and functional genomics of crop plants.. In this study, two contrasting cultivars differencing in their in vitro regeneration efficiency were identified.. Tissue culture friendly cultivar Golden Promise (GP) and tissue culture resistant DWRB91(D91) were selected as contrasting cultivars to investigate the molecular basis of regeneration efficiency through multiomics analysis.

    Polymerase Chain Reaction:

    Article Title: Injury-induced KIF4A neural expression and its role in Schwann cell proliferation suggest a dual function for this kinesin in neural regeneration
    Article Snippet: The Rattus norvegicus Kif4a (NM_001400930.1) and Odc (NM_012615.3) reference sequences were applied to design specific primer pairs (Primer Express 3.0 Software, Applied Biosystems): TCC GGG AGG ATC CTA AGG AA (Kif4a fwd) and GTC CGA GGC AAC TAG CAC AGT (Kif4a rev), TTG ACC AGA TGA ACG GAG TG (Krox20 fwd) and CAG AGA TGG GAG CGA AGC TA (Krox20 rev), GTT CTC GCA GAC CAC CAT CT (Oct6 fwd) and GTC TCC TCC AGC CAC TTG TT (Oct6 rev), GGT TCC AGA GGC CAA ACA TC (Odc fwd) and GTT GCC ACA TTG ACC GTG AC (Odc rev), GAA CGG GAA GCT CAC TGG C (Gapdh fwd) and GCA TGT CAG ATC CAC AAC G (Gapdh rev).

    Article Title: Biosynthesis of Phenolic Compounds of Medicago truncatula After Inoculation with Selected PGPR Strains
    Article Snippet: PCR primers for both semi-quantitative and quantitative methods were designed using Primer Express 3.0 software (Applied Biosystems, Carlsbad, CA, USA)— .

    Article Title: Structural and functional plasticity of metal-responsive transcription factor 1 (MTF-1) within Lophotrochozoa.
    Article Snippet: Control of metal ion metabolism in animals is crucial for their survival.. In this context, the regulation of metallothioneins (MTs), key proteins in metal metabolism and detoxification, by the metal regulatory transcription factor MTF-1 is well established in vertebrates and Drosophila melanogaster.. However, the metal-dependent regulation of MT genes in most other invertebrates remains poorly understood, with MTF-1 so far identified in only a few invertebrate species.

    Article Title: Stress-induced hyperglycemia and expression of glucose cell transport genes in skeletal muscle of critically ill patients: a cross-sectional study
    Article Snippet: Specific primers for IRS1, IRS2, SLC2A1 , and SLC2A4 were designed using published human sequences and Primer Express 3.0 software (Thermo Fisher Scientific). contains the sequence information for all primers.

    Article Title: Transcriptional responses of Metarhizium pingshaense blastospores after UV-B irradiation
    Article Snippet: The primers were designed using Primer Express 3.0 software (Thermo Fisher Scientific, Wilmington, DE, USA) and the transcripts obtained 1 https://www.ncbi.nlm.nih.gov/sra/?term=prjna1127190 Frontiers in Microbiology 04 frontiersin.org from the transcriptome itself (Supplementary Table 1).

    Article Title: Transcriptional responses of Metarhizium pingshaense blastospores after UV-B irradiation
    Article Snippet: The primers were designed using Primer Express 3.0 software (Thermo Fisher Scientific, Wilmington, DE, USA) and the transcripts obtained from the transcriptome itself ( ).

    Article Title: Molecular Biodiversity in Fusarium subglutinans and F. temperatum : A Valuable Tool to Distinguish the Two Sister Species and Determine the Beauvericin Chemotype
    Article Snippet: Species-specific primers were designed using Primer Express 3.0 software (Applied Biosystem, Forster City, CA, USA).

    Article Title: Multi-omics analysis reveals the positive impact of differential chloroplast activity during in vitro regeneration of barley.
    Article Snippet: 1 Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India 2 Institute of Molecular Plant Biology, ETH Zurich, Zurich, Switzerland 3 Department of Chemistry, Biochemistry and Physics, University of Quebec Trois Rivieres, Trois Rivieres, QC, Canada 4 Present address: University Institute of Biotechnology, Chandigarh University, Punjab 140413, India Abstract Existence of potent in vitro regeneration system is a prerequisite for efficient genetic transformation and functional genomics of crop plants.. In this study, two contrasting cultivars differencing in their in vitro regeneration efficiency were identified.. Tissue culture friendly cultivar Golden Promise (GP) and tissue culture resistant DWRB91(D91) were selected as contrasting cultivars to investigate the molecular basis of regeneration efficiency through multiomics analysis.



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